Clostridioides difficile Toxin B/tcdB Recombinant Protein (N-His)
- Catalog number: PV10492
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Clostridioides difficile Toxin B/tcdB Protein, N-His (PV10492) expressed in E. coli, spanning Gly1834-Ile2099. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: His272-Ile381
- Predicted molecular weight: 15.39 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Delta(12)-fatty-acid Desaturase FAD2-like
- Species: Bemisia tabaci
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.
References:
1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489
2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149
3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482
4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933
5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463
6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936
7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356
8. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Clostridioides difficile Toxin B/tcdB Protein, N-His.
