Bacillus cereus gajA Recombinant Protein (N-His)
- Catalog number: PV10619
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Bacillus cereus gajA Protein, N-His (PV10619) expressed in E. coli, spanning Gly258-Gly578. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Gly258-Gly578
- Predicted molecular weight: 39.63 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Endonuclease GajA, 3.1.-.-, Gabija protein GajA, Nicking endonuclease GajA, gajA, IIE_04982
- Species: Bacillus cereus
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Endonuclease GajA is a ~66 kDa protein. Component of antiviral defense system Gabija type I, composed of GajA and GajB. Endonuclease that nicks double-stranded DNA within the sequence 5'-TNNNCGGGNNA-3' in the absence of nucleotides (NTP, dNTP and NDPs), cleaving after C-1. Has no detected ATPase activity. Expression of Gabija type I in B.subtilis (strain BEST7003) confers resistance to phages phi105, phi29, rho14, SpBeta and SBSphiC. Expression of Gabija type I in E.coli B (strain ATCC 11303) confers resistance to phage T7.
References:
1. Doron, S. et al. (2018) Science (New York, N.Y.) 359. PMID: 29371424
2. Cheng, R. et al. (2021) Nucleic acids research 49, 5216-5229. PMID: 33885789
3. Antine, SP. et al. (2024) Nature 625, 360-365. PMID: 37992757 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Bacillus cereus gajA Protein, N-His
