EV71 P2A/Protease 2A Recombinant Protein (N-His)
- Catalog number: PV10763
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant EV71 P2A/Protease 2A Protein, N-His (PV10763) expressed in E. coli, spanning Gly863-Gln1012. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Gly863-Gln1012
- Predicted molecular weight: 18.75 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Genome polyprotein, Protease 2A, P2A, 3.4.22.29, Picornain 2A, Protein 2A
- Species: Human enterovirus 71 (strain 7423/MS/87) (EV71) (EV-71)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Genome polyprotein (P2A/Protease 2A) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization.
References:
1. Zhou, D. et al. (2019) Nature microbiology 4, 414-419. PMID: 30531980 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant EV71 P2A/Protease 2A Protein, N-His
