PEDV S2/Spike glycoprotein 2 Recombinant Protein (N-His & C-His)
- Catalog number: PV10933
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant PEDV S2/Spike glycoprotein 2 Protein, N-His & C-His (PV10933) expressed in E. coli, spanning Met961-Gln1386. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met961-Gln1386
- Predicted molecular weight: 50.80 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Spike glycoprotein, S glycoprotein, E2, Peplomer protein, S
- Species: Porcine epidemic diarrhea virus
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Spike glycoprotein (S2/Spike glycoprotein 2) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with the host receptor, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.
- Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant PEDV S2/Spike glycoprotein 2 Protein, N-His & C-His.
