PEDV Spike glycoprotein (RBD) Recombinant Protein (C-His)
- Catalog number: PV10938
- Availability: In Stock
$298.00
- Ex Tax: $298.00
- Description: Recombinant PEDV Spike glycoprotein (RBD) Protein, C-His (PV10938) expressed in Mammalian Cells, spanning Phe617-Val745. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• Mammalian Expression — Proper folding and native glycosylation.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: Mammalian Cells
- Protein length: Phe617-Val745
- Predicted molecular weight: 16.79 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Spike glycoprotein, S glycoprotein, E2, Peplomer protein, S, RBD
- Species: Porcine epidemic diarrhea virus (strain CV777) (PEDV)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Spike glycoprotein (RBD) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.
- Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant PEDV Spike glycoprotein (RBD) Protein, C-His.
