Mycobacterium tuberculosis esat6 Recombinant Protein (C-His)
- Catalog number: PV11033
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Mycobacterium tuberculosis esat6 Protein, C-His (PV11033) expressed in E. coli, spanning Met1-Ala95. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met1-Ala95
- Predicted molecular weight: 10.73 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: 6 kDa early secretory antigenic target, ESAT-6, esxA, esaT6, Rv3875, MTV027.10
- Species: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: 6 kDa early secretory antigenic target (esat6) is a ~9 kDa protein. A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF production but not IL-2; decreases expression of host ATF-2 and JUN transcription factors by affecting T-cell receptors signaling downstream of ZAP70, without cytotoxicity or apoptosis.
References:
1. Renshaw, PS. et al. (2002) The Journal of biological chemistry 277, 21598-603. PMID: 1194059
2. Sørensen, AL. et al. (1995) Infection and immunity 63, 1710-7. PMID: 7729876
3. Stanley, SA. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 13001-6. PMID: 14557536
4. Andersen, P. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 3359-72. PMID: 7897219
5. Wang, X. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3668-77. PMID: 19265145
6. Peng, H. et al. (2011) The Journal of biological chemistry 286, 24508-18. PMID: 21586573
7. Pathak, SK. et al. (2007) Nature immunology 8, 610-8. PMID: 17486091
8. Mishra, BB. et al. (2010) Cellular microbiology 12, 1046-63. PMID: 20148899
9. Boggaram, V. et al. (2013) The Journal of biological chemistry 288, 25500-25511. PMID: 23867456
10. Sreejit, G. et al. (2014) PLoS pathogens 10, e1004446. PMID: 25356553 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Mycobacterium tuberculosis esat6 Protein, C-His
