WEEV Spike glycoprotein E1 Recombinant Protein (C-His)
- Catalog number: PV11091
- Availability: In Stock
$298.00
- Ex Tax: $298.00
- Description: Recombinant WEEV Spike glycoprotein E1 Protein, C-His (PV11091) expressed in Mammalian Cells, spanning Ala797-Asn1205. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• Mammalian Expression — Proper folding and native glycosylation.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: Mammalian Cells
- Protein length: Ala797-Asn1205
- Predicted molecular weight: 46.93 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Structural polyprotein, p130, Spike glycoprotein E1, E1 envelope glycoprotein
- Species: Western equine encephalitis virus (WEEV)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Structural polyprotein (Spike glycoprotein E1) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.
- Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant WEEV Spike glycoprotein E1 Protein, C-His
