Anti-AIM2 Polyclonal Antibody
- Catalog number: PA10034
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-AIM2 Polyclonal Antibody (PA10034) is a rabbit polyclonal antibody detecting AIM2 in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human AIM2 (Met1-Lys342).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Absent in melanoma 2,Interferon-inducible protein AIM2,AIM2
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Interferon-inducible protein AIM2 is a ~38 kDa protein. Sensor component of the AIM2 inflammasome, which mediates inflammasome activation in response to the presence of double-stranded DNA (dsDNA) in the cytosol, leading to subsequent pyroptosis. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. Acts as a recognition receptor (PRR): specifically recognizes and binds dsDNA in the cytosol, and mediates the formation of the inflammasome polymeric complex composed of AIM2, CASP1 and PYCARD/ASC. Recruitment of pro-caspase-1 (proCASP1) to the AIM2 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion. In some cells, CASP1 activation mediates cleavage and activation of GSDMD, triggering pyroptosis without promoting cytokine secretion.
References:
1. Woerner, SM. et al. (2007) Genes, chromosomes & cancer 46, 1080-9. PMID: 1772670
2. Hornung, V. et al. (2009) Nature 458, 514-8. PMID: 19158675
3. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13. PMID: 19158676
4. Bürckstümmer, T. et al. (2009) Nature immunology 10, 266-72. PMID: 19158679
5. Tsuchiya, K. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 1186-95. PMID: 20566831
6. Jin, T. et al. (2013) The Journal of biological chemistry 288, 13225-35. PMID: 23530044
7. Morrone, SR. et al. (2015) Nature communications 6, 7827. PMID: 26197926
8. Lu, A. et al. (2015) Cell discovery 1, 15013-. PMID: 26583071
9. Matyszewski, M. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1963-E1972. PMID: 29440442
10. Matyszewski, M. et al. (2021) Nature communications 12, 2735. PMID: 33980849 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-AIM2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: Jurkat cell lysate
Datasheet
