Anti-DBH Polyclonal Antibody

Anti-DBH Polyclonal Antibody

  • Catalog number: PA10369
  • Availability: In Stock
  • $168.00

  • Ex Tax: $168.00


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  • Description: Anti-DBH Polyclonal Antibody (PA10369) is a rabbit polyclonal antibody detecting DBH in ELISA, IHC, WB. Suitable for Human, Bovine, and Equus caballus.
    Highlights:
    • Affinity Purified — Minimal background and high purity for reliable results.
    • Multi-Application — Validated across multiple applications.
    • Multi-Species — Cross-reactive for translational research.
  • Host species: Rabbit
  • Species reactivity: Human, Mouse
  • Immunogen: E. coli - derived recombinant Human DBH (His335-Val571).
  • Form: Liquid
  • Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
  • Clonality: Polyclonal
  • Isotype: IgG
  • Applications: ELISA, IHC, WB
  • Target: Dopamine beta-hydroxylase,DBH,Dopamine beta-monooxygenase
  • Purification: Purified by antigen affinity column.
  • Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
  • Background: Dopamine beta-hydroxylase (DBH) is a ~69 kDa protein. Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.
  • Note: For research use only.

Images

  • Immunofluorescence for Anti-DBH Polyclonal Antibody
    Immunofluorescence

    Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting DBH (HY186014) at a concentration of 11.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of DBH was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

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