Anti-NQO1 Polyclonal Antibody
- Catalog number: PA10502
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-NQO1 Polyclonal Antibody (PA10502) is a rabbit polyclonal antibody detecting NQO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Pongo abelii, and Rat.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications.
• Multi-Species — Cross-reactive for translational research. - Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Immunogen: E. coli - derived recombinant Human NQO1 (Met1-Lys274).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: NAD(P)H:quinone oxidoreductase 1,Azoreductase,Phylloquinone reductase,NQO1,DT-diaphorase,NAD(P)H dehydrogenase [quinone] 1,QR1,DIA4,NMOR1,Quinone reductase 1,Menadione reductase,DTD
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: NAD(P)H dehydrogenase [quinone] 1 (NQO1) is a ~30 kDa protein. Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion.
References:
1. Chen, S. et al. (1997) The Journal of biological chemistry 272, 1437-9. PMID: 8999809
2. Siegel, D. et al. (1997) Molecular pharmacology 52, 300-5. PMID: 9271353
3. Siegel, D. et al. (2004) Molecular pharmacology 65, 1238-47. PMID: 15102952
4. Asher, G. et al. (2005) Genes & development 19, 316-21. PMID: 15687255
5. Medina-Carmona, E. et al. (2017) Scientific reports 7, 44532. PMID: 28291250 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-NQO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lystae
Lane 2: LO2 cell lysate
Lane 3: A549 cell lysate
Datasheet
