Anti-GM130/GOLGA2 Polyclonal Antibody
- Catalog number: PA11001
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-GM130/GOLGA2 Polyclonal Antibody (PA11001) is a rabbit polyclonal antibody detecting GOLGA2 in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human GM130/GOLGA2 (Ala361-Ala678).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: 130 kDa cis-Golgi matrix protein,GM130,GM130 autoantigen,Golgin-95,Golgin subfamily A member 2,GOLGA2
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Golgin subfamily A member 2 (GM130/GOLGA2) is a ~113 kDa protein. Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization.
References:
1. Puthenveedu, MA. et al. (2006) Nature cell biology 8, 238-48. PMID: 16489344
2. Wei, JH. et al. (2015) Cell 162, 287-299. PMID: 2616594
3. Rivero, S. et al. (2009) The EMBO journal 28, 1016-28. PMID: 1924249
4. Kodani, A. et al. (2008) Molecular biology of the cell 19, 745-53. PMID: 18045989
5. Kodani, A. et al. (2009) Molecular biology of the cell 20, 1192-200. PMID: 19109421
6. Marra, P. et al. (2007) Molecular biology of the cell 18, 1595-608. PMID: 17314401 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-GOLGA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K562 cell lysate
