Anti-BECN1 Polyclonal Antibody
- Catalog number: PA11077
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-BECN1 Polyclonal Antibody (PA11077) is a rabbit polyclonal antibody detecting BECN1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications.
• Multi-Species — Cross-reactive for translational research. - Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Immunogen: E. coli - derived recombinant Human BECN1 (Gln13-Gln239).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: BECN1,Beclin-1,Coiled-coil myosin-like BCL2-interacting protein,Protein GT197,GT197
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Beclin-1 (BECN1) is a ~51 kDa protein. Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis.
References:
1. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871
2. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617
3. Margariti, A. et al. (2013) The Journal of biological chemistry 288, 859-72. PMID: 23184933
4. Xia, P. et al. (2013) The EMBO journal 32, 2685-96. PMID: 23974797
5. Xu, C. et al. (2014) Autophagy 10, 2239-50. PMID: 25484083
6. Ashkenazi, A. et al. (2017) Nature 545, 108-111. PMID: 2844546
7. Wyatt, S. et al. (2023) Biochemistry 62, 2934-2951. PMID: 37776275
8. Sagona, AP. et al. (2010) Nature cell biology 12, 362-71. PMID: 2020853
9. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123
10. Liu, K. et al. (2016) The Journal of cell biology 212, 181-98. PMID: 26783301 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-BECN1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: Mouse testis lysate
Lane 3: Mouse brain lysate
Lane 4: Mouse lung lysate
Lane 5: Rat testis lysate
Datasheet
