Anti-CD326/EPCAM Polyclonal Antibody
- Catalog number: PA11504
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-CD326/EPCAM Polyclonal Antibody (PA11504) is a rabbit polyclonal antibody detecting EPCAM in ELISA, IHC, WB, IF. Suitable for Human and Macaca mulatta.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Immunogen: E. coli - derived recombinant Human CD326/EPCAM (Gln24-Lys265).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Epithelial glycoprotein 314,hEGP314,Major gastrointestinal tumor-associated protein GA733-2,EGP314,TACSTD1,Adenocarcinoma-associated antigen,TROP1,EPCAM,Epithelial glycoprotein,KS 1/4 antigen,Tumor-associated calcium signal transducer 1,M4S1,CD326,Ep-CAM,EGP,Epithelial cell adhesion molecule,M1S2,KSA,Cell surface glycoprotein Trop-1,GA733-2,Epithelial cell surface antigen,MIC18
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Epithelial cell adhesion molecule (CD326/EPCAM) is a ~34 kDa protein. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
- Note: For research use only.
Images
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ImmunofluorescenceHela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting EpCAM (HB104014) at a concentration of 20.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of EpCAM was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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Western blotWestern blot analysis was performed using anti-EPCAM polyclonal antibody at 1μg/ml on various samples.
Lane 1: HCT116 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Mouse colon lysate
Datasheet
