Anti-Human RBM15 Polyclonal Antibody
- Catalog number: PA11868
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Human RBM15 Polyclonal Antibody (PA11868) is a rabbit polyclonal antibody detecting RBM15 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human RBM15 (Thr386-Thr529).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: RBM15, RNA-binding motif protein 15, OTT1, One-twenty two protein 1, OTT, RNA-binding protein 15
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: RNA-binding protein 15 (RBM15) is a ~107 kDa protein. RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex. Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist. Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation.
References:
1. Patil, DP. et al. (2016) Nature 537, 369-373. PMID: 27602518
2. Zhang, L. et al. (2015) eLife 4. PMID: 26575292
3. Lindtner, S. et al. (2006) The Journal of biological chemistry 281, 36915-28. PMID: 17001072
4. Zolotukhin, AS. et al. (2009) Nucleic acids research 37, 7151-62. PMID: 19786495
5. Mercher, T. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 5776-9. PMID: 11344311 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-RBM15 polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: K562 cell lysate
Lane 3: Hela cell lysate
Lane 4: 293T cell lysate
Lane 5: Jurkat cell lysate
Datasheet
