Anti-Human RSAD2 Polyclonal Antibody
- Catalog number: PA12028
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human RSAD2 (Ser74-Trp361).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: RSAD2, Viperin, Cytomegalovirus-induced gene 5 protein, Radical S-adenosyl methionine domain-containing protein 2, Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible, CIG5
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: S-adenosylmethionine-dependent nucleotide dehydratase RSAD2 is a ~42 kDa protein. Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism. In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication. Therefore, inhibits a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), zika virus, and human immunodeficiency virus (HIV-1). Also promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6.
References:
1. Yuan, Y. et al. (2020) Molecular cell 77, 734-747.e7. PMID: 3181235
2. Gizzi, AS. et al. (2018) Nature 558, 610-614. PMID: 29925952
3. Dumbrepatil, AB. et al. (2019) The Journal of biological chemistry 294, 6888-6898. PMID: 30872404
4. Wei, C. et al. (2018) Viruses 11. PMID: 30587778
5. Vanwalscappel, B. et al. (2019) International journal of molecular sciences 20. PMID: 30934824
6. Li, M. et al. (2019) Frontiers in immunology 10, 2810. PMID: 31921110 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-RSAD2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HUVEC cell lysate
Lane 2: Mouse spleen lysate
Lane 3: Mouse brain lysate
Lane 4: Mouse lung lysate
Lane 5: Rat spleen lysate
Lane 6: Rat brain lysate
Lane 7: Rat lung lysate
Datasheet
