Anti-Human IPO4 Polyclonal Antibody
- Catalog number: PA12052
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human IPO4 (Asn667-Ser1081).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: RanBP4, Imp4b, Importin-4b, Ran-binding protein 4, RANBP4, IPO4, Importin-4, Imp4, IMP4B
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Importin-4 (IPO4) is a ~118 kDa protein. Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
References:
1. Jäkel, S. et al. (2002) The EMBO journal 21, 377-86. PMID: 1182343
2. Miyauchi, Y. et al. (2005) The Journal of biological chemistry 280, 40901-8. PMID: 16207705
3. Pradeepa, MM. et al. (2008) Molecular and cellular biology 28, 4331-41. PMID: 17682055
4. Alvarez, F. et al. (2011) The Journal of biological chemistry 286, 17714-21. PMID: 21454524
5. Apta-Smith, MJ. et al. (2018) The EMBO journal 37. PMID: 30177573 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-IPO4 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Lncap cell lysate
Lane 3: A549 cell lysate
Datasheet
