Anti-Human NME1 Polyclonal Antibody
- Catalog number: PA12064
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human NME1 (Met1-Glu152).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: NDPKA, NME1, NDP kinase A, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, NM23, Metastasis inhibition factor nm23, GAAD, Tumor metastatic process-associated protein, NDK A, NM23-H1
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Nucleoside diphosphate kinase A (NME1) is a ~17 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism. Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins. May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair.
References:
1. Wagner, PD. et al. (2000) The Journal of biological chemistry 275, 35570-6. PMID: 10952986
2. Ma, D. et al. (2004) The Journal of biological chemistry 279, 18073-84. PMID: 14960567
3. Yoon, JH. et al. (2005) Biochemistry 44, 15774-86. PMID: 16313181
4. Gilles, AM. et al. (1991) The Journal of biological chemistry 266, 8784-9. PMID: 1851158
5. Kim, MS. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 669-80. PMID: 23519676
6. Yu, BYK. et al. (2021) Redox biology 44, 101978. PMID: 3390307
7. MacDonald, NJ. et al. (1996) The Journal of biological chemistry 271, 25107-16. PMID: 8810265
8. Freije, JM. et al. (1997) The Journal of biological chemistry 272, 5525-32. PMID: 9038158
9. Engel, M. et al. (1995) European journal of biochemistry 234, 200-7. PMID: 8529641 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-NME1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse lung lysate
Lane 3: Mouse liver lysate
Lane 4: Rat brain lysate
Lane 5: Rat lung lysate
Lane 6: Rat liver lysate
Datasheet
