Anti-Human FEN1 Polyclonal Antibody

Size

50 µg

100 µg (+$110.00)

Qty

Host species: Rabbit
Species reactivity: Human
Immunogen: E. coli - derived recombinant Human FEN1 (Met1-Thr336).
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Clonality: Polyclonal
Isotype: IgG
Applications: ELISA, IHC, WB
Target: FEN-1, Maturation factor 1, hFEN-1, DNase IV, RAD2, FEN1, Flap endonuclease 1, MF1, Flap structure-specific endonuclease 1
Purification: Purified by antigen affinity column.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background: Flap endonuclease 1 (FEN1) is a ~42 kDa protein. Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions.
Note: For research use only.

Datasheet

$168.00