Anti-Human NFS1 Polyclonal Antibody
- Catalog number: PA12261
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human NFS1 (Arg72-His457).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: NFS1, NIFS, Cysteine desulfurase, mitochondrial
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Cysteine desulfurase (NFS1) is a ~50 kDa protein. Cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex, leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU. The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1. After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner.
References:
1. Marelja, Z. et al. (2008) The Journal of biological chemistry 283, 25178-25185. PMID: 18650437
2. Boniecki, MT. et al. (2017) Nature communications 8, 1287. PMID: 29097656
3. Fox, NG. et al. (2019) Nature communications 10, 2210. PMID: 31101807 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-NFS1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: Mouse heart lysate
Lane 3: Mouse kidney lysate
Lane 4: Mouse brain lysate
Lane 5: Rat kidney lysate
Datasheet
