Anti-PRKCQ Polyclonal Antibody
- Catalog number: PA12818
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Immunogen: E. coli - derived recombinant Human PRKCQ (Phe380-Phe634).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: nPKC-theta, PRKCQ, PRKCT, Protein kinase C theta type
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Protein kinase C theta type (PRKCQ) is a ~81 kDa protein. Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling.
References:
1. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608
2. López-Sánchez, I. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 5510-5. PMID: 23509302
3. Malik, AU. et al. (2022) The Biochemical journal 479, 1941-1965. PMID: 36040231 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-PRKCQ polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Datasheet
