Anti-CD247/CD3Z Polyclonal Antibody
- Catalog number: PA13218
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human, Mouse, Rat
- Immunogen: E. coli - derived recombinant Human CD247/CD3Z (Arg52-Arg164).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: CD3Z, CD247, TCRZ, T-cell receptor T3 zeta chain, T3Z, T-cell surface glycoprotein CD3 zeta chain
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: T-cell surface glycoprotein CD3 zeta chain (CD247/CD3Z) is a ~18 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD247/CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme.
References:
1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049
2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158
3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098
4. Iwashima, M. et al. (1994) Science (New York, N.Y.) 263, 1136-9. PMID: 7509083 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-CD247 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Datasheet
