Anti-Human ANO1 Polyclonal Antibody
- Catalog number: PA13651
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human ANO1 (Met117-Gly325).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Anoctamin-1, Tumor-amplified and overexpressed sequence 2, Transmembrane protein 16A, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, TMEM16A, ANO1, TAOS2, ORAOV2, DOG1
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Anoctamin-1 (ANO1/TMEM16A) is a ~114 kDa protein. Calcium-activated chloride channel (CaCC). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression.
References:
1. Schreiber, R. et al. (2010) The Journal of biological chemistry 285, 7838-45. PMID: 20056604
2. Ousingsawat, J. et al. (2011) Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 28, 715-24. PMID: 22178883
3. Tian, Y. et al. (2012) Journal of cell science 125, 4991-8. PMID: 22946059
4. Park, JH. et al. (2021) Journal of medical genetics 58, 247-253. PMID: 32487539
5. Benedetto, R. et al. (2017) Scientific reports 7, 12397. PMID: 28963502
6. Cabrita, I. et al. (2021) American journal of respiratory cell and molecular biology 64, 50-58. PMID: 33026825
7. Simões, FB. et al. (2019) Life science alliance 2. PMID: 31732694 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-ANO1 polyclonal antibody at 1µg/ml on various samples.
Lane 1: 293T cell lysate
Datasheet
