Anti-Human MUL1 Polyclonal Antibody
- Catalog number: PA13700
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human MUL1 (Lys44-Gln224).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Mitochondrial-anchored protein ligase, E3 ubiquitin-protein ligase MUL1, Protein Hades, C1orf166, RNF218, Mitochondrial ubiquitin ligase activator of NFKB 1, Growth inhibition and death E3 ligase, MUL1, RING finger protein 218, MAPL, RING-type E3 ubiquitin transferase NFKB 1, Putative NF-kappa-B-activating protein 266, GIDE, MULAN, E3 SUMO-protein ligase MUL1
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Mitochondrial ubiquitin ligase activator of NFKB 1 (MUL1) is a ~39 kDa protein. Exhibits weak E3 ubiquitin-protein ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteasomal degradation. Mediates polyubiquitination of cytoplasmic TP53 at 'Lys-24' which targets TP53 for proteasomal degradation, thus reducing TP53 levels in the cytoplasm and mitochondrion. Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations.
References:
1. Zhang, B. et al. (2008) Cell research 18, 900-10. PMID: 18591963
2. Braschi, E. et al. (2009) EMBO reports 10, 748-54. PMID: 1940783
3. Bae, S. et al. (2012) Cell research 22, 873-85. PMID: 22410793
4. Jung, JH. et al. (2011) Cell death and differentiation 18, 1865-75. PMID: 21597459
5. Neuspiel, M. et al. (2008) Current biology : CB 18, 102-8. PMID: 18207745
6. Yun, J. et al. (2014) eLife 3, e01958. PMID: 24898855 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-MUL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse skeletal muscle lysate
Lane 2: Rat skeletal muscle lysate
Datasheet
