Anti-Human U2AF2 Polyclonal Antibody
- Catalog number: PA14004
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Human U2AF2 Polyclonal Antibody (PA14004) is a rabbit polyclonal antibody detecting U2AF2 in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human U2AF2 (Pro138-Gly338).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Splicing factor U2AF 65 kDa subunit, hU2AF65, hU2AF(65), U2 auxiliary factor 65 kDa subunit, U2AF65, U2AF2, U2 snRNP auxiliary factor large subunit
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Splicing factor U2AF 65 kDa subunit (U2AF2) is a ~53 kDa protein. Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly.
References:
1. Millevoi, S. et al. (2006) The EMBO journal 25, 4854-64. PMID: 17024186
2. David, CJ. et al. (2011) Genes & development 25, 972-83. PMID: 21536736 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-U2AF2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Datasheet
