Anti-Mouse CD143/ACE Polyclonal Antibody
- Catalog number: PA14153
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Mouse CD143/ACE Polyclonal Antibody (PA14153) is a rabbit polyclonal antibody detecting CD143 in ELISA, IHC, WB. Suitable for Mouse.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Mouse
- Immunogen: E. coli - derived recombinant Mouse CD143/ACE (Ser45-Asp300).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Dipeptidyl carboxypeptidase I, DCP, CD143, Angiotensin-converting enzyme, DCP1, ACE, Kininase II
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Angiotensin-converting enzyme (CD143/ACE) is a ~150 kDa protein. Dipeptidyl carboxypeptidase that removes dipeptides from the C-terminus of a variety of circulating hormones, such as angiotensin I, bradykinin or enkephalins, thereby playing a key role in the regulation of blood pressure, electrolyte homeostasis or synaptic plasticity. Composed of two similar catalytic domains, each possessing a functional active site, with different selectivity for substrates. Plays a major role in the angiotensin-renin system that regulates blood pressure and sodium retention by the kidney by converting angiotensin I to angiotensin II, resulting in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator, and therefore enhance the blood pressure response. Acts as a regulator of synaptic transmission by mediating cleavage of neuropeptide hormones, such as substance P, neurotensin or enkephalins.
References:
1. Kessler, SP. et al. (2002) The Journal of biological chemistry 277, 4271-6. PMID: 11723129
2. Kessler, SP. et al. (2003) The Journal of biological chemistry 278, 21105-12. PMID: 12777443
3. Fuchs, S. et al. (2004) The Journal of biological chemistry 279, 15946-53. PMID: 14757757
4. Fuchs, S. et al. (2005) Nature medicine 11, 1140-2; author reply 1142-3. PMID: 16270063
5. Trieu, BH. et al. (2022) Science (New York, N.Y.) 375, 1177-1182. PMID: 35201898
6. Krege, JH. et al. (1995) Nature 375, 146-8. PMID: 775317
7. Esther, CR. et al. (1996) Laboratory investigation; a journal of technical methods and pathology 74, 953-65. PMID: 864279
8. Tian, B. et al. (1997) Hypertension (Dallas, Tex. : 1979) 30, 128-33. PMID: 9231832
9. Junot, C. et al. (2001) The Journal of pharmacology and experimental therapeutics 297, 606-11. PMID: 11303049 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-ACE polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse lung lysate
