Anti-Human MT-ATP8 Polyclonal Antibody
- Catalog number: PA14428
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Human MT-ATP8 Polyclonal Antibody (PA14428) is a rabbit polyclonal antibody detecting MT-ATP8 in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human MT-ATP8 (Gln25-Ser68).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: ATP synthase protein 8, A6L, F-ATPase subunit 8, MT-ATP8, ATP8, ATPASE8, MTATP8
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: ATP synthase F(0) complex subunit 8 (MT-ATP8) is a ~7 kDa protein. Subunit 8, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.
References:
1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256 - Note: For research use only.
Images
-
Western blotWestern blot analysis was performed using anti-MT-ATP8 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Lncap cell lysate
Lane 3: A549 cell lysate
Lane 4: Hela cell lysate
