Anti-Human BUB3 Polyclonal Antibody
- Catalog number: PA14613
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Human BUB3 Polyclonal Antibody (PA14613) is a rabbit polyclonal antibody detecting BUB3 in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human BUB3 (Met1-Thr328).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Mitotic checkpoint protein BUB3, BUB3
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Mitotic checkpoint protein BUB3 is a ~37 kDa protein. Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20.
- Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-BUB3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Datasheet
