Anti-Human METTL3 Polyclonal Antibody
- Catalog number: PA15171
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Human METTL3 Polyclonal Antibody (PA15171) is a rabbit polyclonal antibody detecting METTL3 in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human METTL3 (Met1-Leu580).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: METTL3, N6-adenosine-methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase catalytic subunit, MTA70, Methyltransferase-like protein 3, MT-A70, hMETTL3
- Purification: Purified by antigen affinity column.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: N(6)-adenosine-methyltransferase catalytic subunit METTL3 is a ~64 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs.
References:
1. Dominissini, D. et al. (2012) Nature 485, 201-6. PMID: 2257596
2. Wang, X. et al. (2014) Nature 505, 117-20. PMID: 24284625
3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671
4. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998
5. Alarcón, CR. et al. (2015) Cell 162, 1299-308. PMID: 2632168
6. Meyer, KD. et al. (2015) Cell 163, 999-1010. PMID: 26593424
7. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194
8. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 273733379. Śledź, P. et al. (2016) eLife 5. PMID: 27627798
9. Xiang, Y. et al. (2017) Nature 543, 573-576. PMID: 28297716 - Note: For research use only.
Images
-
Western blotWestern blot analysis was performed using anti-METTL3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
