Anti-Human TIGAR Polyclonal Antibody
- Catalog number: PA15203
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Human TIGAR Polyclonal Antibody (PA15203) is a rabbit polyclonal antibody detecting TIGAR in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human TIGAR (Met1-Arg270).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5, TP53-induced glycolysis regulatory phosphatase
- Purification: Antigen affinity purification.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Fructose-2,6-bisphosphatase TIGAR is a ~30 kDa protein. Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3.
References:
1. Li, H. et al. (2009) The Journal of biological chemistry 284, 1748-54. PMID: 19015259
2. Bensaad, K. et al. (2006) Cell 126, 107-20. PMID: 1683988
3. Wanka, C. et al. (2012) The Journal of biological chemistry 287, 33436-46. PMID: 22887998
4. Bensaad, K. et al. (2009) The EMBO journal 28, 3015-26. PMID: 19713938
5. Cheung, EC. et al. (2013) Developmental cell 25, 463-77. PMID: 23726973
6. Ye, L. et al. (2013) Biochemical and biophysical research communications 437, 300-6. PMID: 2381704
7. Cheung, EC. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 20491-6. PMID: 23185017 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-TIGAR polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: HepG2 cell lysate
Datasheet
