Anti-Human ULK1 Polyclonal Antibody
- Catalog number: PA15307
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Human ULK1 Polyclonal Antibody (PA15307) is a rabbit polyclonal antibody detecting ULK1 in ELISA, IHC, WB. Suitable for Human.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Human
- Immunogen: E. coli - derived recombinant Human ULK1 (Met1-His275).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, KIAA0722, Serine/threonine-protein kinase ULK1, hATG1, ATG1
- Purification: Antigen affinity purification.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Serine/threonine-protein kinase ULK1 is a ~112 kDa protein. Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences.
References:
1. Chan, EY. et al. (2009) Molecular and cellular biology 29, 157-71. PMID: 18936157
2. Löffler, AS. et al. (2011) Autophagy 7, 696-706. PMID: 21460634
3. Jung, CH. et al. (2011) Autophagy 7, 1212-21. PMID: 21795849
4. Nazio, F. et al. (2013) Nature cell biology 15, 406-16. PMID: 23524951
5. Ro, SH. et al. (2014) The FEBS journal 281, 3816-27. PMID: 25040165
6. Kim, JH. et al. (2018) EMBO reports 19. PMID: 29487085
7. Di Rienzo, M. et al. (2019) Science advances 5, eaau8857. PMID: 31123703 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-ULK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Datasheet
