Anti-Bovine ISG15 Polyclonal Antibody
- Catalog number: PA15412
- Availability: In Stock
$168.00
- Ex Tax: $168.00
- Description: Anti-Bovine ISG15 Polyclonal Antibody (PA15412) is a rabbit polyclonal antibody detecting ISG15 in ELISA, IHC, WB. Suitable for Bovine.
Highlights:
• Affinity Purified — Minimal background and high purity for reliable results.
• Multi-Application — Validated across multiple applications. - Host species: Rabbit
- Species reactivity: Bovine
- Immunogen: E. coli - derived recombinant Bovine ISG15 (Met1-Gly154).
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
- Clonality: Polyclonal
- Isotype: IgG
- Applications: ELISA, IHC, WB
- Target: Interferon-induced 17 kDa protein, G1P2, hUCRP, Ubiquitin-like protein ISG15, IP17, UCRP, ISG15, Ubiquitin cross-reactive protein, Interferon-induced 15 kDa protein,
- Purification: Antigen affinity purification.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Background: Ubiquitin-like protein ISG15 is a ~17 kDa protein. Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Exhibits antiviral activity towards both DNA and RNA viruses. The secreted form of ISG15 can: induce natural killer cell proliferation, augment lymphokine-activated-killer (LAK) activity, induce dendritic cell maturation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10; the interaction is mediated by ITGAL.
References:
1. Johnson, GA. et al. (1998) Biology of reproduction 58, 898-904. PMID: 9546718 - Note: For research use only.
Images
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Western blotWestern blot analysis was performed using anti-ISG15 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
