Anti-VSV NP/Nucleoprotein Antibody (1014)

Description: Anti-VSV NP/Nucleoprotein Antibody (1014) [1014] (RA10238) is a human monoclonal antibody detecting Nucleoprotein in FCM, IP.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications.
Host species: Human
Isotype: IgG1, kappa
Species reactivity: Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: FCM, IP
Target: NP, Nucleoprotein, Nucleocapsid protein, Protein N
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Nucleoprotein (NP) is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.
References:
1. Green, TJ. et al. (2006) Science (New York, N.Y.) 313, 357-60. PMID: 16778022
2. Ge, P. et al. (2010) Science (New York, N.Y.) 327, 689-93. PMID: 20133572
3. Gumpper, RH. et al. (2018) Journal of virology 92. PMID: 2943797
4. Jenni, S. et al. (2022) Nature communications 13, 4802. PMID: 35970826
5. Zhou, K. et al. (2022) Nature communications 13, 5980. PMID: 3621693
6. Green, TJ. et al. (2011) Journal of virology 85, 2714-22. PMID: 21177817
7. Si, Z. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2111948119. PMID: 35476516
Note: For research use only.