Anti-VSV G/Glycoprotein G Antibody (VI22)

Description: Anti-VSV G/Glycoprotein G Antibody (VI22) [VI22] (RA10253) is a human monoclonal antibody detecting Glycoprotein G in ELISA.
Host species: Human
Isotype: IgG1, kappa
Species reactivity: Vesicular stomatitis Indiana virus (strain Mudd-Summers) (VSIV)
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: G, Glycoprotein G
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Glycoprotein G is a ~57 kDa protein. Attaches the virus to host LDL receptors, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane.
References:
1. Nikolic, J. et al. (2018) Nature communications 9, 1029. PMID: 29531262
2. Roche, S. et al. (2006) Science (New York, N.Y.) 313, 187-91. PMID: 16840692
3. Roche, S. et al. (2007) Science (New York, N.Y.) 315, 843-8. PMID: 17289996
Note: For research use only.