Anti-Mouse HMGB1 Antibody (DPH1.1)

Host species: Rat
Isotype: IgG1, kappa
Species reactivity: Human, Mouse, Rat, Bovine, Dog, Horse, Pig
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: FCM, FuncS, IF, WB
Target: High mobility group protein B1, HMGB1, HMG1, HMG-1, High mobility group protein 1
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: High mobility group protein B1 (HMGB1) is a ~24 kDa protein. Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as a sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy.
References:
1. Yang, H. et al. (2013) Journal of leukocyte biology 93, 865-73. PMID: 23446148
2. Li, G. et al. (2013) Frontiers in immunology 4, 68. PMID: 23519706
3. Li, G. et al. (2013) Seminars in cancer biology 23, 380-90. PMID: 23994764
4. Lee, SA. et al. (2014) Yonsei medical journal 55, 1165-76. PMID: 25048472
5. Mitola, S. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 12-5. PMID: 16365390
Note: For research use only.