Anti-Mouse TP53/p53 Antibody (PAb242)

Description: Anti-Mouse TP53/p53 Antibody (PAb242) [PAb242] (RA10531) is a rat monoclonal antibody detecting TP53 in ELISA, IF, IHC, IP, RIA, WB. Suitable for Mouse.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Rat
Isotype: IgG2a, kappa
Species reactivity: Mouse
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, IF, IHC, IP, RIA, WB
Target: Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Cellular tumor antigen p53 (TP53) is a ~43 kDa protein. Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.
References:
1. Allton, K. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 11612-6. PMID: 19556538
2. Huarte, M. et al. (2010) Cell 142, 409-19. PMID: 2067399
3. Vaseva, AV. et al. (2012) Cell 149, 1536-48. PMID: 2272644
4. Miki, T. et al. (2013) Nature communications 4, 2444. PMID: 24051492
Note: For research use only.