Anti-Mouse CXCL10/IP-10 Antibody (54)

Description: Anti-Mouse CXCL10/IP-10 Antibody (54) [54] (RA10539) is a rat monoclonal antibody detecting IP-10 in ELISA, FCM. Suitable for Human and Mouse.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications.
Host species: Rat
Isotype: IgG1, kappa
Species reactivity: Human, Mouse
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 0.75 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, FCM
Target: Gamma-IP10, C-X-C motif chemokine 10, INP10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, SCYB10, IP-10, CXCL10
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: C-X-C motif chemokine 10 (CXCL10/IP-10) is a ~10 kDa protein. Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site.
References:
1. Gao, N. et al. (2017) Angiogenesis 20, 505-518. PMID: 28623423
2. Lindell, DM. et al. (2008) European journal of immunology 38, 2168-79. PMID: 18624292
3. Wuest, TR. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 7985-93. PMID: 1901799
4. Srivastava, R. et al. (2017) Journal of virology 91. PMID: 28468883
5. Rappert, A. et al. (2004) The Journal of neuroscience : the official journal of the Society for Neuroscience 24, 8500-9. PMID: 15456824
Note: For research use only.