Anti-Mouse IL2 Antibody (JES6-1A12)

Description: Anti-Mouse IL2 Antibody (JES6-1A12) [JES6-1A12] (RA10543) is a rat monoclonal antibody detecting IL2 in ELISA, FCM. Suitable for Mouse.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications.
Host species: Rat
Isotype: IgG2b, kappa
Species reactivity: Mouse
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, FCM
Target: IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Interleukin-2 (IL2) is a ~19 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.
References:
1. Zhu, J. et al. (2003) Immunity 19, 739-48. PMID: 1461486
2. Reya, T. et al. (1998) Developmental immunology 5, 287-302. PMID: 9814585
3. Smith, GA. et al. (2016) Nature chemical biology 12, 373-9. PMID: 27018889
Note: For research use only.