Anti-Phospho-Smad2 (pT8) Antibody (SAA0289)

Description: Anti-Phospho-Smad2 (pT8) Antibody (SAA0289) [SAA0289] (RA10611) is a mouse monoclonal antibody detecting SMAD2 in IHC, IP, WB. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1.98 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: IHC, IP, WB
Target: Mothers against decapentaplegic homolog 2, SMAD 2, SMAD2, JV18-1, Mad-related protein 2, hMAD-2, MADR2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, Smad2, hSMAD2, MAD homolog 2
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: SMAD family member 2 (p-Smad2) is a ~52 kDa protein. Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma.
References:
1. Eppert, K. et al. (1996) Cell 86, 543-52. PMID: 8752209
Note: For research use only.