Anti-Phospho-POLR2A-CTD (p(YSPTSPSYSPTSPS)) Antibody (S7)

Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: IHC, IP, WB
Target: DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, POLR2A, POLR2, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: DNA-directed RNA polymerase II subunit RPB1 (p-POLR2A) is a ~217 kDa protein. Catalytic core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA.
References:
1. Kassube, SA. et al. (2013) Nature structural & molecular biology 20, 892-9. PMID: 2374838
2. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
3. Jishage, M. et al. (2018) Nature structural & molecular biology 25, 859-867. PMID: 30190596
4. Kershnar, E. et al. (1998) The Journal of biological chemistry 273, 34444-53. PMID: 9852112
5. Vo Ngoc, L. et al. (2017) Genes & development 31, 6-11. PMID: 28108474
6. Wang, D. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 843-7. PMID: 8381534
7. Yu, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8608-13. PMID: 26124092
Note: For research use only.