Anti-Phospho-HTRA2 (pS212) Antibody (P6.C12)

Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: IHC, IP, WB
Target: High temperature requirement protein A2, OMI, HtrA2, PRSS25, Serine protease 25, Serine proteinase OMI, Serine protease HTRA2, mitochondrial, HTRA2, Omi stress-regulated endoprotease
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Serine protease HTRA2, mitochondrial (pHTRA2) is a ~48 kDa protein. Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6. Cleaves THAP5 and promotes its degradation during apoptosis.
References:
1. Savopoulos, JW. et al. (2000) Protein expression and purification 19, 227-34. PMID: 10873535
2. Bartke, T. et al. (2004) Molecular cell 14, 801-11. PMID: 15200957
3. Hunkeler, M. et al. (2023) Science (New York, N.Y.) 379, 1105-1111. PMID: 36758104
4. Ehrmann, JF. et al. (2023) Science (New York, N.Y.) 379, 1117-1123. PMID: 36758105
5. Balakrishnan, MP. et al. (2009) American journal of physiology. Heart and circulatory physiology 297, H643-53. PMID: 19502560
Note: For research use only.