Anti-Phospho-CASC5 (pT943) Antibody (pMELT-KNL1)

Description: Anti-Phospho-CASC5 (pT943) Antibody (pMELT-KNL1) [pMELT-KNL1] (RA10653) is a mouse monoclonal antibody detecting Protein CASC5 in IHC, IP, WB. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 2 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: IHC, IP, WB
Target: Blinkin, CT29, Kinetochore-null protein 1, Cancer/testis antigen 29, Bub-linking kinetochore protein, Protein CASC5, Protein D40/AF15q14, ALL1-fused gene from chromosome 15q14 protein, Kinetochore scaffold 1, CASC5, KNL1, Cancer susceptibility candidate gene 5 protein, KIAA1570, AF15q14
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Outer kinetochore KNL1 complex subunit KNL1 (p-KNL1/CASC5) is a ~265 kDa protein. Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
References:
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2. Kiyomitsu, T. et al. (2007) Developmental cell 13, 663-676. PMID: 17981135
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5. Kiyomitsu, T. et al. (2011) Molecular and cellular biology 31, 998-1011. PMID: 21199919
6. Bolanos-Garcia, VM. et al. (2011) Structure (London, England : 1993) 19, 1691-700. PMID: 22000412
7. Krenn, V. et al. (2012) The Journal of cell biology 196, 451-67. PMID: 22331848
8. Petrovic, A. et al. (2016) Cell 167, 1028-1040.e15. PMID: 27881301
9. Bajaj, R. et al. (2018) Structure (London, England : 1993) 26, 1327-1336.e4. PMID: 30100357
10. Yatskevich, S. et al. (2024) Nature structural & molecular biology 31, 874-883. PMID: 38459127
Note: For research use only.