Anti-Human NONO Reference Antibody (PAT-LM1)

Description: Anti-Human NONO (PAT-LM1) (BS11772) is a research-grade recombinant antibody targeting 54 kDa nuclear RNA- and DNA-binding protein. Produced in mammalian cells with native-like glycosylation.
Highlights:
• Research Grade — For PK/PD studies, assay development, and ADA research.
• Native Glycosylation — Mammalian expression ensures native-like patterns.
Host species: Human
Isotype: IgG
Species reactivity: Human
Immunogen:
Form: Liquid
Storage instructions:
Storage buffer: 0.01M PBS, pH7.4.
Concentration: 1mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: Research Grade Biosimilar
Target: 54kDanuclearRNA-andDNA-bindingprotein,NRB54,p54nrb,NMT55,NonOprotein,p54(nrb),55kDanuclearprotein,NONO,DNA-bindingp52/p100complex,52kDasubunit,Non-POUdomain-containingoctamer-bindingprotein
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: <1EU/mg, determined by LAL gel clotting assay
Expression system: Mammalian Cells
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names: PAT-LM1,PATLM1,PATLM1
Background: Non-POU domain-containing octamer-binding protein (NONO) is a ~54 kDa protein. DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b.
References:
1. Zhang, Z. et al. (2001) Cell 106, 465-75. PMID: 11525732
2. Peng, R. et al. (2002) RNA (New York, N.Y.) 8, 1334-47. PMID: 1240347
3. Mircsof, D. et al. (2015) Nature neuroscience 18, 1731-6. PMID: 26571461
4. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728
Note: For research use only. Not suitable for clinical or therapeutic use.