Anti-Human Integrin alpha V/CD51 Reference Antibody (Abituzumab, RUO)
- Catalog number: BS10137
- Availability: In Stock
$176.00
- Ex Tax: $176.00
Highlights:
• Research Grade — For PK/PD studies, assay development, and ADA research.
• Native Glycosylation — Mammalian expression ensures native-like patterns.
Host species: Humanized
Isotype: IgG2-kappa
Species reactivity: Human
Immunogen:
Form: Liquid
Storage instructions:
Storage buffer: 0.01M PBS, pH7.4.
Concentration: 1mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: Research Grade Biosimilar
Target: MSK8,VNRA,Vitronectinreceptor,Integrinalpha-V,Vitronectinreceptorsubunitalpha,CD51,ITGAV,VTNR
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: <1EU/mg, determined by LAL gel clotting assay
Expression system: Mammalian Cells
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names: DI-17E6,EMD525797,CAS:1105038-73-0
Background: Integrin alpha-V (CD51/ITGAV) is a ~116 kDa protein. The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling.
References:
1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415
2. Ieguchi, K. et al. (2010) The Journal of biological chemistry 285, 31388-98. PMID: 20682778
3. Mori, S. et al. (2008) The Journal of biological chemistry 283, 18066-75. PMID: 18441324
4. Mori, S. et al. (2017) Bioscience reports 37. PMID: 28302677
5. Saegusa, J. et al. (2009) The Journal of biological chemistry 284, 24106-14. PMID: 19578119
6. Cedano Prieto, DM. et al. (2017) PloS one 12, e0184285. PMID: 28873464
7. Takada, YK. et al. (2017) The Journal of biological chemistry 292, 20067-20075. PMID: 2903043
8. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536
9. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877
10. Bax, DV. et al. (2003) The Journal of biological chemistry 278, 34605-16. PMID: 12807887
Note: For research use only. Not suitable for clinical or therapeutic use.
Images
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BioactivitySEC-HPLC detection for Research Grade Abituzumab.
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Flow CytometryFlow-cytometry using anti-human CD51 antibody. A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD51 monoclonal antibody (Catalog HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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SDS-PAGESDS-PAGE for Research Grade Abituzumab.
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Flow CytoMetryFlow-cytometry using APC anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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Flow CytoMetryFlow-cytometry using PE anti-human CD51 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD51 monoclonal antibody (Catalog: HY065016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
