Anti-Human RHO/Rhodopsin Antibody (K42-41L)

Description: Anti-Human RHO/Rhodopsin Antibody (K42-41L) [K42-41L] (RA11784) is a mouse monoclonal antibody detecting Rhodopsin in Blocking, ELISA, ICC. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: Blocking, ELISA, ICC
Target: Rhodopsin, OPN2, Opsin-2, RHO
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Rhodopsin is a ~38 kDa protein. Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
References:
1. Sieving, PA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 880-4. PMID: 7846071
2. Rao, VR. et al. (1994) Nature 367, 639-42. PMID: 8107847
3. Noorwez, SM. et al. (2003) The Journal of biological chemistry 278, 14442-14450. PMID: 12566452
4. Dryja, TP. et al. (1990) The New England journal of medicine 323, 1302-7. PMID: 2215617
5. Kang, Y. et al. (2015) Nature 523, 561-7. PMID: 26200343
6. He, Y. et al. (2017) Cell research 27, 728-747. PMID: 28524165
7. Zhou, XE. et al. (2017) Cell 170, 457-469.e13. PMID: 28753425
Note: For research use only.