InVivoMAb Anti-Human ACE2 Antibody (3E8#)

Description: InVivoMAb Anti-Human ACE2 Antibody (3E8#) [3E8#] (RA12037) is a mouse monoclonal antibody detecting Angiotensin-converting enzyme homolog in ELISA, FCM, Neutralization. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, FCM, Neutralization
Target: Angiotensin-converting enzyme homolog, ACE2, ACEH, Metalloprotease MPROT15, Angiotensin-converting enzyme-related carboxypeptidase, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Angiotensin-converting enzyme 2 (ACE2) is a ~92 kDa protein. Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity.
References:
1. Wang, W. et al. (2016) Hypertension (Dallas, Tex. : 1979) 68, 365-77. PMID: 27217402
2. Tipnis, SR. et al. (2000) The Journal of biological chemistry 275, 33238-43. PMID: 10924499
3. Donoghue, M. et al. (2000) Circulation research 87, E1-9. PMID: 10969042
4. Vickers, C. et al. (2002) The Journal of biological chemistry 277, 14838-43. PMID: 11815627
5. Zisman, LS. et al. (2003) Circulation 108, 1707-12. PMID: 14504186
6. Rushworth, CA. et al. (2008) The FEBS journal 275, 6033-42. PMID: 19021774
7. Kowalczuk, S. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 2880-7. PMID: 18424768
8. Camargo, SM. et al. (2009) Gastroenterology 136, 872-82. PMID: 19185582
Note: For research use only. Not suitable for clinical or therapeutic use.