Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51)

Description: Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51) [KZ51] (RA12130) is a human monoclonal antibody detecting Nucleoprotein in ELISA, IF.
Host species: Human
Isotype: IgG
Species reactivity: Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, IF
Target: NP, Nucleoprotein, Nucleocapsid protein
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.
References:
1. Johnson, RF. et al. (2006) Virology journal 3, 31. PMID: 16719918
2. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894
3. Kirchdoerfer, RN. et al. (2015) Cell reports 12, 140-149. PMID: 26119732
4. Sugita, Y. et al. (2018) Nature 563, 137-140. PMID: 30333622
5. Wan, W. et al. (2017) Nature 551, 394-397. PMID: 29144446
6. Beniac, DR. et al. (2012) PloS one 7, e29608. PMID: 22247782
7. Kirchdoerfer, RN. et al. (2016) PLoS pathogens 12, e1005937. PMID: 27755595
Note: For research use only.