Anti-Human ROBO1 Antibody (SAA1494)

Description: Anti-Human ROBO1 Antibody (SAA1494) [SAA1494] (RA12138) is a mouse monoclonal antibody detecting ROBO1 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: H-Robo-1, DUTT1, Roundabout homolog 1, Deleted in U twenty twenty, ROBO1
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Roundabout homolog 1 (ROBO1) is a ~180 kDa protein. Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.
References:
1. Brose, K. et al. (1999) Cell 96, 795-806. PMID: 10102268
2. Leyva-Díaz, E. et al. (2014) Current biology : CB 24, 494-508. PMID: 24560577
3. Kong, R. et al. (2015) The Journal of clinical investigation 125, 4407-20. PMID: 26529257
Note: For research use only.