Anti-Human IDE Antibody (SAA1582)

Description: Anti-Human IDE Antibody (SAA1582) [SAA1582] (RA12226) is a mouse monoclonal antibody detecting IDE in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: Insulysin, IDE, Abeta-degrading protease, Insulin protease, Insulinase, Insulin-degrading enzyme
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Insulin-degrading enzyme (IDE) is a ~117 kDa protein. Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin. Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP. Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP.
References:
1. Vekrellis, K. et al. (2000) The Journal of neuroscience : the official journal of the Society for Neuroscience 20, 1657-65. PMID: 10684867
2. Shen, Y. et al. (2006) Nature 443, 870-4. PMID: 17051221
3. Im, H. et al. (2007) The Journal of biological chemistry 282, 25453-63. PMID: 17613531
4. Malito, E. et al. (2008) Biochemistry 47, 12822-34. PMID: 18986166
5. Manolopoulou, M. et al. (2009) The Journal of biological chemistry 284, 14177-88. PMID: 19321446
6. Ralat, LA. et al. (2011) The Journal of biological chemistry 286, 4670-9. PMID: 21098034
7. Affholter, JA. et al. (1990) Molecular endocrinology (Baltimore, Md.) 4, 1125-35. PMID: 2293021
8. McCord, LA. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 13827-32. PMID: 2392239
9. Maianti, JP. et al. (2014) Nature 511, 94-8. PMID: 24847884
10. Deprez-Poulain, R. et al. (2015) Nature communications 6, 8250. PMID: 26394692
Note: For research use only.