Anti-Human PTN Antibody (SAA1600)

Description: Anti-Human PTN Antibody (SAA1600) [SAA1600] (RA12244) is a mouse monoclonal antibody detecting PTN in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: Heparin-binding neurite outgrowth-promoting factor 1, Heparin-binding brain mitogen, PTN, HBGF-8, Pleiotrophin, Heparin-binding growth factor 8, HBBM, HBNF-1, NEGF1, Osteoblast-specific factor 1, HBNF, HBNF1, Heparin-binding growth-associated molecule, HB-GAM, OSF-1, Heparin-binding neurite outgrowth-promoting factor
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Pleiotrophin (PTN) is a ~18 kDa protein. Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway.
References:
1. Stoica, GE. et al. (2001) The Journal of biological chemistry 276, 16772-9. PMID: 1127872
2. Fukada, M. et al. (2006) FEBS letters 580, 4051-6. PMID: 16814777
3. Mikelis, C. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 1459-69. PMID: 1914153
4. Ryan, E. et al. (2016) The FEBS journal 283, 1488-503. PMID: 26896299
5. Kuboyama, K. et al. (2016) The Journal of biological chemistry 291, 18117-28. PMID: 27445335
6. Wellstein, A. et al. (1992) The Journal of biological chemistry 267, 2582-7. PMID: 1733956
7. Kretschmer, PJ. et al. (1991) Growth factors (Chur, Switzerland) 5, 99-114. PMID: 1768439
8. Imai, S. et al. (2009) Bone 44, 785-94. PMID: 19442624
Note: For research use only.