Anti-Human KEAP1 Antibody (SAA1674)

Description: Anti-Human KEAP1 Antibody (SAA1674) [SAA1674] (RA12318) is a mouse monoclonal antibody detecting KEAP1 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: INrf2, INRF2, KLHL19, KEAP1, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, Kelch-like protein 19
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Kelch-like ECH-associated protein 1 (KEAP1) is a ~69 kDa protein. Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation.
References:
1. Zhang, DD. et al. (2003) Molecular and cellular biology 23, 8137-51. PMID: 14585973
2. Strachan, GD. et al. (2004) Biochemistry 43, 12113-22. PMID: 1537955
3. Zhang, DD. et al. (2004) Molecular and cellular biology 24, 10941-53. PMID: 15572695
4. Furukawa, M. et al. (2005) Molecular and cellular biology 25, 162-71. PMID: 15601839
5. Zhang, DD. et al. (2005) The Journal of biological chemistry 280, 30091-9. PMID: 15983046
6. Cai, C. et al. (2023) Nature communications 14, 3648. PMID: 37339955
7. Eggler, AL. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 10070-5. PMID: 16006525
8. Gao, L. et al. (2007) The Journal of biological chemistry 282, 2529-37. PMID: 17127771
9. Eggler, AL. et al. (2009) The Biochemical journal 422, 171-80. PMID: 19489739
Note: For research use only.